Composite

Part:BBa_K5477030

Designed by: Isaiah Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)


XRE-pMEL1-NanoLuc reporter module

The XRE-pMEL1-NanoLuc reporter module is a component of the AhR (Aryl hydrocarbon receptor) biosensor device, designed to detect and report the presence of environmental toxins, such as polycyclic aromatic hydrocarbons (PAHs), dioxins and polychlorinated biphenyls (PCBs) upon ligand-induced binding of AhR-ARNT heterodimer to Xenobiotic response element (XRE) (1) (2). In this module, the XRE was used upstream of the pMEL1 promoter that drives the expression of the NanoLuc reporter gene.

The pUUS plasmid is a multi-copy plasmid used in yeast, carrying a URA3 marker for selection in uracil auxotrophic strains. In the system, pUUS was utilized in the reporter module, enabling the expression of the reporter gene, NanoLuc BBa_K1680009. This composite part was used in these devices: BBa_K5477041 and BBa_K5477042.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 59
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 59
    Illegal BamHI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Carambia, A., Schuran, F.A. The aryl hydrocarbon receptor in liver inflammation. Semin Immunopathol 43, 563–575 (2021). https://doi.org/10.1007/s00281-021-00867-8

2. Mandal A, Biswas N, Alam MN. Implications of xenobiotic-response element(s) and aryl hydrocarbon receptor in health and diseases. Hum Cell. 2023 Sep;36(5):1638-1655. doi: 10.1007/s13577-023-00931-5. Epub 2023 Jun 17. PMID: 37329424.

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